New techniques in RNA sequencing, spatial tanscriptomics and proteomics have rapidly developed and allow for assessment of near complete gene expression at a microscopic lesional, and cellular level, focused on a precise area of interest making it possible to compare gene expression between different types of pulmonary vascular cells under baseline and disease conditions. This symposium will present novel findings from multiple laboratories demonstrating how these techniques are moving the field of pulmonary vascular biology forward, with a focus on methodology development, the required expertise to carry out such an analyses, bioinformatics, and interpretation of the data.
• Describe new technologies that allow assessment of gene expression at the cellular and lesional level from animals and patients with pulmonary vascular disease
• Apply new technologies for assessing cell specific gene expression to advance basic science research in pulmonary vascular biology
• Apply new knowledge competence to better understand the pathophysiology of pulmonary vascular disease to help guide the diagnosis and treatment of pulmonary arterial hypertension
Naftali Kaminski, MD, ATSF
Soni Pullamsetti, PhD
Jason Hong, MD, PhD
Alexander Brodsky, PhD
Integrated Analysis of scRNAseq Data Sets to Develop a Comprehensive Atlas of Endothelial Gene Expression in the Normal Lung
Reactivation of Transcriptional Programs That Orchestrate Fetal Lung Development in Human Pulmonary Hypertension
The Use of Single-Cell Transcriptomics in Animal Models of Pulmonary Hypertension to Identify Genetic Changes Relevant to Human Pulmonary Hypertension
Combining Spatial Transcriptomics and Single Cell RNAseq to Examine Gene Expression in Pulmonary Vasculature Endothelial Cells